Coding

Part:BBa_K4728003:Design

Designed by: Michelle Li   Group: iGEM23_Queens-Canada   (2023-10-08)


TphA2 (Large subunit of oxygenase component)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part is codon optimized for Escherichia coli.


Source

TphA2 comes from the species Comamonas sp. strain E6.

References

Sasoh, M., Masai, E., Ishibashi, S., Hara, H., Kamimura, N., Miyauchi, K., & Fukuda, M. (2006). Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Applied and environmental microbiology, 72(3), 1825–1832. https://doi.org/10.1128/AEM.72.3.1825-1832.2006